Bayern, which would have remained top even with a loss, matched its 52-year-old record of 43 consecutive rounds atop the league.
Michael Olise scored twice and set up three more goals as Bayern Munich came from two down to beat Freiburg 6-2 at home in the Bundesliga on Saturday.
Harry Kane scored as Bayern Munich won 2-1 at Freiburg on Saturday to move six points clear of Bayer Leverkusen, who threw away a two-goal lead to draw 2-2 at RB Leipzig.
Bayern Munich striker Harry Kane will hope to overcome a slump in front of goal when his side travel to face Freiburg on Saturday.
The gap at the top of the Bundesliga table is down to four points between Bayern Munich and Bayer Leverkusen.
Union Berlin goalkeeper Frederik Ronnow saved a first-half penalty in a scoreless home draw against Freiburg in the German capital on Friday.
Bayern face Freiburg, who have won only once in 17 previous meetings.
Florian Wirtz scored after 84 seconds as Bayer Leverkusen made it 38 games unbeaten in all competitions with a 3-2 victory in Freiburg on Sunday.
Bayern Munich were held to a 2-2 draw at Freiburg on Friday, an 87th-minute equaliser from Lucas Hoeler giving leaders Bayer Leverkusen a chance to go 10 points clear.
Donyell Malen scored a first-half brace as Borussia Dortmund eased to a 3-0 home victory over Freiburg in the Bundesliga on Friday
Kevin Schade joined the Premier League club on loan from Bundesliga side Freiburg in January and has now signed a five-year contract
Bayern, 20-time German Cup winners, have now been eliminated before the semis for three straight years since their treble-winning season in 2019-20.
Bayern Munich goalkeeper Manuel Neuer will miss his side's top-of-the-table clash with Bundesliga high-fliers Freiburg on Sunday due to ongoing problems with his shoulder, coach Julian Nagelsmann confirmed
Union battled to a well-earned 1-1 draw at home to Bayern, allowing fellow high-fliers Freiburg to leapfrog their way into first after they beat Bayer Leverkusen in a five-goal thriller.
German club Freiburg on Monday lodged an appeal against their 4-1 defeat against Bayern Munich in the Bundesliga.
Borussia Dortmund slumped to a shock 2-1 defeat at Freiburg, their first in 11 years, as star striker Erling Braut Haaland drew a rare Bundesliga blank.
Borussia Dortmund's Champions League qualification hopes suffered a blow with a 2-1 loss at Freiburg in the Bundesliga.
Borussia Moenchengladbach, enjoying their best season since 1984, remain in fourth place - the final Champions League qualifying spot - on 56 points.