Captain Joe Root and Alastair Cook both fell cheaply before the tourists reached 196 for four when bad light ended an attritional, rain-affected day.
Tim Paine dropped England batsman James Vince on Day 1 of the 1st Ashes Test at The Gabba in Brisbane on Thursday.
Jake Ball has played four Tests for England but is yet to play an Ashes Test.
If either Steve Smith or Joe Root struggle for runs, it is liable to damage their team's prospects and add to the pressure that confronts most captains in an Ashes series.
Graeme Swann and Adam Gilchrist will be on the same commentary team for the Ashes series.
England will be without Ben Stokes for the first Ashes Test in Brisbane, with the 26-year-old made unavailable for selection by country's board "until further notice".
Steve Smith said injuries were "part and parcel of playing cricket" and that David Warner's condition had improved a "fair bit".
As always, the Ashes series is preceded by a lot of verbal duels.
Lyon recalled the devastation caused by left-armer Mitchell Johnson in 2013-2014, when he took 37 wickets.
The opening batsman injured his neck while taking a high catch on the Gabba outfield, and later left for the dressing room to get treatment.
Ben Stokes took to Twitter and launched a scathing attack on Matthew Hayden for his comments on the England squad.
Australia have enlisted Usain Bolt to boost their running between the wickets during the Ashes series against England.
Bayliss said England got everything they wanted out of the three warm-up games and was confident of defying their poor record at the Gabba when the First Test begins on Thursday.
Tim Paine was chosen for his first Test in seven years and 34-year-old Shaun Marsh was recalled to the Test side for the eighth time in controversial selections announced on Friday.
Novices Jason Sangha and Matt Short scored their maiden first-class centuries for a Cricket Australia XI to frustrate England on the last day of their final Ashes warm-up game on Saturday.
Shane Warne pointed to Tim Paine's inclusion in the Australia squad, saying that they're picking wicketkeepers that aren't even keeping for their state.
The recall of wicketkeeper Tim Paine to the Australia Ashes 2017 squad after seven years came as a big surprise.
Alastair Cook took 11 balls to get off the mark but once set he hardly looked in trouble until he edged an attempted cut off Matt Short to the keeper on 70.