Cristiano Ronaldo scored two goals in four first half minutes and had a third ruled offside by VAR as Portugal crushed Switzerland 4-0 in Lisbon.
Ahead of this year's FIFA World Cup, England are scheduled to face Switzerland in a friendly fixture.
Italy will have to get through the play-offs if they are to return to the World Cup after a goalless draw at Northern Ireland on Monday allowed Switzerland to pip them on the final day of qualifying.
Harry Kane scored a first half hat-trick as England romped towards the 2022 World Cup with a 5-0 thrashing of Albania, but Italy still have work to do to reach Qatar after a 1-1 draw with Switzerland.
Chiellini's absence is a big blow heading into Friday's clash in Rome against the Swiss, who trail Group C leaders Italy on goal difference with two games remaining for both teams.
Euro 2020: As the European Championship was held across 11 cities in 11 countries, some teams played more games at home than the others.
Spain defeated Switzerland 3-1 on penalties to seal a place in the semi-final of the ongoing Euro 2020 on Friday.
UEFA Euro 2020: Spain's men's football team, led by captain Sergio Busquets, is expected to face a resilient Swiss side in the first quarter-final match today.
Switzerland will try to follow up their shock elimination of world champions France at Euro 2020 against Spain on Friday after reaching the quarter-finals of a major tournament for the first time in 67 years.
France had not lost a competitive match since a qualifier against Turkey in June 2019, but finished on the losing side in their first shootout since the 2006 World Cup final against Italy.
Euro 2020: Switzerland beat France on penalties in the Round of 16 to progress to the quarter-finals.
World Cup holders France are in Euro 2020 action on Monday as they face Switzerland in Bucharest looking to secure a place in the quarter-finals, while Luka Modric leads Croatia into battle against Spain.
Euro 2020: Switzerland beat Turkey 3-1 in their Group A clash on Sunday but they will wait to see if they reach Round of 16 as one of the four best third-placed teams.
Euro 2020: Italy qualified for the round of 16 as a Manuel Locatelli brace helped secure a 3-0 win against Switzerland.
Euro 2020: Italy will take on Switzerland in their second Group A match in the ongoing Euro 2020 at the Stadio Olimpico in Rome
Kieffer Moore scored the equaliser for Wales as they secured a 1-1 draw against Switzerland in a Group A Euro 2020 clash on Saturday.
Sergio Busquets sustained the injury on November 15 while on international duty with Spain, during the Nations League match against Switzerland.
UEFA Nations League: Sergio Ramos was making his 177th appearance for Spain to become the most capped European player in history but it was a night to forget for the defender.