Barcelona's first slip-up since returning from the three-month hiatus hands Real Madrid the chance to regain top spot in La Liga this weekend.
Sevilla, who sit third in La Liga, hold a five-point lead over fifth-placed Getafe, although most of their closest rivals still have to play in this round of matches.
Lucas Ocampos gave Sevilla the lead from a 56th-minute penalty and Fernando Reges then added the second goal for the home side, who are third in the table.
There have been reports of Nelson Semedo breaching health protocol while four Sevilla players were seen attending a barbecue.
Fireworks, banners, plumes of smoke and crowds, delirious at the sight of a team bus, let alone a goal, the Seville derby is a fixture renowned for its intensity and cherished by its supporters.
The first round of games following the coronavirus shutdown will be played over four days, with leaders Barcelona at Mallorca on June 13 and Real Madrid at home to Eibar the next day.
Barcelona successfully bounced back from Saturday's surprise La Liga loss away at Valencia by hammering Leganes 5-0 in the Copa del Rey.
Barcelona's defeat to Valencia raised early doubts about Quique Setien's appointment and gave Real Madrid the chance to move three points clear at the top.
Real Madrid climbed above Barcelona to top La Liga on Saturday as Casemiro scored twice in a gritty 2-1 win over Sevilla.
Barcelona are second in the La Liga table, two points behind Real Madrid, following their 4-0 win over Sevilla at Camp Nou.
La Liga: Real Madrid are now four points clear of Barcelona after five games played.
Alejandro Pozo delivered the late dagger in 10-man Sevilla's 2-1 friendly win over Liverpool in Boston Sunday
Liverpool face Spanish side Sevilla on Sunday at Boston's Fenway Park baseball stadium in the second game of their three-match US tour.
Jose Antonio Reyes won a European under-21 title with Spain in 2002 and went on to play 21 times for Spain between 2003 and 2006, scoring four goals.
Lionel Messi leads the La Liga scoring charts after notching his 25th goal in 23 league appearances this season.
Lionel Messi made sure that Ernesto Valverde's team kept their place at the top of the pile with a mesmerising display.
Victory for Barcelona took them back to the top of La Liga, in front of Sevilla and Atletico Madrid, who were each held to 1-1 draws.