Zinedine Zidane has said Gareth Bale told him he did not want to play for Real Madrid against Manchester City in the Champions League last 16 on Friday.
Zinedine Zidane named 24 players on Wednesday for the trip to Manchester.
After scoring Real Madrid's third goal in the 37th minute, Marcelo dropped onto his left knee and raised his clinched right fist into the air.
Gareth Bale's agent says the Real Madrid forward is unlikely to return to the Premier League because he wants to finish his career at the Bernabeu.
Gareth Bale said there should be no rush to restart La Liga during the coronavirus lockdown.
Barcelona can cut the gap back to three points by beating Levante at home on Sunday but Atletico Madrid now trail leaders Real Madrid by 13 points.
Eden Hazard could be sidelined for more than a month after suffering a fracture in his ankle while Marcelo might also miss the game against Barcelona, after he sustained a calf strain in training.
Real Madrid face PSG at the Santiago Bernabeu, with Gareth Bale's future in doubt and his standing among the fans perhaps never lower.
Wales managed a 1-1 with World Cup runners-up Croatia on Sunday which left their Euro 2020 qualifying hopes on a knife-edge.
Real Madrid vs Osasuna: Eden Hazard and Gareth Bale were among a number of players rested by coach Zinedine Zidane, with Real Madrid facing seven games in 21 days.
Zinedine Zidane said in July it would be "best for everyone" if Gareth Bale left this summer but it was the Welshman's double that earned Real Madrid a point.
Zinedine Zidane said he believes Gareth Bale will now stay, with the Spanish transfer window closing on September 2.
Zinedine Zidane has made clear that Gareth Bale is not in his plans as he looks to rejuvenate Real Madrid following their miserable season last term.
Gareth Bale has already been told by Real Madrid coach Zinedine Zidane that he does not form part of his future plans at the Spanish giants.
Real Madrid clawed back from two goals down to finish full time at 2-2, and prevailed 3-2 in the penalty shootout dictated by the International Champions Cup exhibition tournament format.
Real Madrid boss Zinedine Zidane said at the weekend the La Liga giants were poised to offload Gareth Bale.
"I did not disrespect anyone, least of all Gareth," a frustrated-sounding Zinedine Zidane said.
Zinedine Zidane said the club were working on Gareth Bale's departure, adding that he hoped it happens soon, "for everyone's sake".