Serena Williams' bid for her 24th Grand Slam title was brought to a shuddering halt by Karolina Pliskova.
Serena Williams is gunning to match Margaret Court's record of singles majors and claim an eighth Australian Open crown.
Former world number one Karolina Pliskova booked her spot in the semi-finals for the second straight year having also beaten Wozniacki earlier in the tournament.
Karolina Pliskova needed just 63 minutes to complete a clinical 6-4, 6-4 victory over Naomi Osaka in Tokyo.
Six-time champion Serena Williams shook off a sluggish start to power past eighth-seeded Karolina Pliskova 6-4, 6-3 on Tuesday and into the US Open semi-finals.
Maria Sharapova's last win over Serena Williams came at the 2004 WTA Tour Championships -- before even the birth of Twitter and YouTube.
Maria Sharapova will return to the showpiece Court Philippe Chatrier for the first time while Rafael Nadal defends his 15-0 record over Richard Gasquet.
Karolina Pliskova rallied from a set down to beat fellow Czech and 20th seed Barbora Strycova 6-7 (5/7), 6-3, 6-2 and make the Australian Open quarter-finals.
World number one Rafael Nadal notched up his 50th win at the US Open on Monday to reach the quarter-finals for the seventh time.
Rafael Nadal and Roger Federer won their third round matches to move closer to a blockbuster semi-final clash at the US Open.
Switzerland's Federer will be seeking his 20th Grand Slam title and his sixth in New York.
Australia's Nick Kyrgios humbled new world number one Rafael Nadal 6-2, 7-5 to reach Cincinnati Masters semi-finals on Friday.
Five tennis players have a chance to grab the world No 1 ranking at this week's Cincinnati Open.
Roger Federer climbed to No 3 in the latest ATP singles rankings while Karolina Pliskova topped the WTA charts despite never having won a Major.
Karolina Pliskova became the firm Wimbledon favourite on Wednesday when compatriot and two-time champion Petra Kvitova was knocked out.
Novak Djokovic clinched his first title since January with a 6-3, 6-4 victory over Gael Monfils.
With the Fed Cup tie level at 2-2, US Open finalist Karolina Pliskova teamed up with Barbora Strycova to defeat French Open doubles champions Caroline Garcia and Kristina Mladenovic 7-5, 7-5 to prolong the Czech Republic's reign
Karolina Pliskova beat Kristina Mladenovic 6-3, 4-6, 16-14 in an epic rubber which lasted 12 minutes short of four hours as the Czechs, chasing a fifth title in six years, got off to a sensational start.