John Isner will play the No. 1 singles spot for the U.S. team against Brazil in the first round of Davis Cup World Group matches.
Team captain Jim Courier made the announcement on Thursday at the draw ceremony.
Isner has not played competitively since withdrawing from his opening-round match at the APIA International Sydney three weeks ago with a knee injury.
Sam Querrey will play the No. 2 position. Mike and Bob Bryan will be the doubles team.
The Brazilian team will consist of singles players Thomaz Bellucci and Thiago Alves and the doubles team of Marcelo Melo and Bruno Soares. Querrey will play Bellucci in Friday's opening match followed by the Isner-Alves match.
The three-day event begins Friday at the Jacksonville Veterans Memorial Arena.