Valencia has won all three Champions League matches since its opening-round loss to Bayern Munich, and the Spanish side would like to keep the momentum when last season's runner-up visits on Tuesday.
Bayern comes with the knowledge that it has lost only once in eight encounters against Valencia, with four draws. It beat the Spanish side on penalties to lift the Champions League trophy in 2001.
But Valencia is unbeaten in its last eight matches at home in the competition.
Neither team will secure advancement if BATE Borisov beats Lille in the other Group F match. Lille must win by a greater margin than 3-1 to have any hope of overtaking the Belarusian team for third place.