Zinedine Zidane confirmed that Eden Hazard is feeling pain again in his troublesome right ankle and will miss Real Madrid's game away at Athletic Bilbao in La Liga on Sunday.
Real Madrid registered an impressive victory over Valencia that reduces their deficit to two points behind Barcelona, who play away at Sevilla on Friday.
Real Madrid secured a 3-1 win against Eibar at the Estadio Alfredo Di Stefano on Sunday.
After scoring Real Madrid's third goal in the 37th minute, Marcelo dropped onto his left knee and raised his clinched right fist into the air.
Eden Hazard was looking beyond Real Madrid to the Euros after undergoing surgery on his right foot in March while some felt Luis Suarez had played his last game for Barcelona after having a knee operation in January.
Roberto Martinez said that there were no complications with Eden Hazard's surgery and that the Real Madrid star is "not far from running again".
Real Madrid did not announce a recovery time for Eden Hazard but his latest setback will be a worry for Belgium boss Roberto Martinez ahead of Euro 2020 this summer.
Real Madrid's nightmare afternoon was made worse by another injury to Eden Hazard, who was forced off in the second half of their 0-1 defeat to Levante.
Eden Hazard's comeback also coincided with Real Madrid conceding twice in a league game for the first time since October, even if they did let in three in the Copa del Rey against Real Sociedad two weeks ago.
Barcelona were beaten 0-1 by Athletic Bilbao at San Mames while Real Madrid lost 3-4 in a seven-goal thriller to Real Sociedad at the Santiago Bernabeu.
Barcelona can cut the gap back to three points by beating Levante at home on Sunday but Atletico Madrid now trail leaders Real Madrid by 13 points.
If Eden Hazard returns against Sevilla in La Liga on January 18 he will have been out for almost eight weeks.
Eden Hazard could be sidelined for more than a month after suffering a fracture in his ankle while Marcelo might also miss the game against Barcelona, after he sustained a calf strain in training.
Eden Hazard had shown glimpses of his best form in recent weeks after a slow start in Spain, in part due to a thigh strain he picked up at the beginning of the season.
Lionel Messi delivered an incredible dead-ball hat-trick on Saturday to steer Barcelona to a 4-1 victory over Celta Vigo.
Euro 2020: Romelu Lukaku took his record international tally past 50 goals as Belgium opened up an unassailable 11-point lead over third-placed Cyprus.
Eden Hazard had become a growing concern after five underwhelming matches, the patience granted to him after his injury in pre-season beginning to run out after his 100 million euros move from Chelsea.