Bayern Munich were held to a 2-2 draw at Freiburg on Friday, an 87th-minute equaliser from Lucas Hoeler giving leaders Bayer Leverkusen a chance to go 10 points clear.
A Bayern victory will allow Thomas Tuchel's side to leapfrog Leverkusen into first spot, while becoming the first team to inflict defeat on their rivals who are 30 games unbeaten this season.
Jamal Musiala scored twice and Harry Kane added a late goal as Bayern Munich won 3-0 at home over Hoffenheim on Friday
Teenage Bayern Munich striker Mathys Tel struck late as his side came from behind to beat FC Copenhagen 2-1 in their Champions League clash in the Danish capital on Tuesday.
Jamal Musiala has established himself as a member of the starting lineup with Bayern and Germany in recent seasons.
Dortmund scored two second half goals to draw the game but the point was not enough, Bayern winning the title on goal difference.
Bayern Munich's teenage forward Jamal Musiala laid on two assists to help his side extend their lead atop the table to six points in a 2-0 win away at Schalke on Saturday
Bayern Munich manager Julian Nagelsmann lauded his "fun to watch" side as they reclaimed top spot on the table with a dominant 6-2 home defeat of Mainz
Borussia Dortmund host Bayern Munich in the Bundesliga on Saturday with arguably the two sides' most important players, Jude Bellingham and Jamal Musiala, firing on all cylinders
Bundesliga: A masterclass from Jamal Musiala, as well as a first goal in five games for Sadio Mane, helped Bayern Munich thump hapless Bayer Leverkusen 4-0 at home