Sania Mirza was ousted from the Indian Wells WTA Premier tournament after suffering a straight set defeat against world number 26 Anabel Medina Garrigues, here.
Sania, a wild card entrant, lost 2-6 5-7 to Spain's 24th seed in the second round.
Sania had beaten German qualifier Kristina Barrios in the opening round 4-6 6-2 6-4.
Sania's challenge is still alive in the doubles event with Russian partner Elena Vesnina.
The second seeds have reached second round after beating Vera Dushevina and Shahar Peer 7-5 6-2.
They face Timea Bacsinszky and Alberta Brianti in the second round.