There was controversy over VAR as Everton held Manchester United to a 1-1 draw at Goodison Park.
Goals from Virgil Van Dijk and Mohamed Salah gave Liverpool a 2-0 win over rival Manchester United.
Premiers League: Already struggling Manchester United were dealt another huge blow as their two key players Paul Pogba and David de Gea were ruled out of the Liverpool clash.
David de Gea, who had been linked with a return to Spain with Real Madrid for several seasons, has put pen to paper on a new four-year deal with Manchester United.
Paul Pogba, who has been linked with a move to Juventus and Real Madrid, starred in Manchester United's dominant first-half.
David De Gea is in the last year of his contract and has been linked with French champions Paris Saint-Germain.
David de Gea is so far holding out in contract negotiations to prolong his current deal beyond the summer of 2020.
Jose Mourinho said he wanted David de Gea to stay, and he remains optimistic an agreement on the player's will be reached.
Spain face a tough challenge to reach the FIFA World Cup 2018 knockout as the 2010 champions take on eliminated Morocco in their Group B clash at Kaliningrad Stadium on Monday.
Spain would be desperately seeking three points in their World Cup Group B match with Iran after being held by Portugal.
Iran are sitting on top of the World Cup Group B team standings and Spain would like to change that.
De Gea had previously denied any involvement in the sex assault case
David de Gea deflected the attention away from Paul Pogba with another spectacular display of goalkeeping as Manchester United came away from their Champions League last 16, first leg against Sevilla in Spain with a 0-0 draw on Wednesday.
The Spain goalkeeper, who almost joined Real Madrid in 2015 and continues to be linked with a move there, has a contract that expires in June 2019.
Spain were frustrated throughout by the Czechs' stubborn defensive game, which finally fell apart when Gerard Pique got his head to Andres Iniesta's cross towards the back post where he beat goalkeeper Petr Cech in the 87th minute
David De Gea has strongly denied allegations that he organised a party at which two women were forced to have sex with two footballers
Spain will be aiming for a hat-trick of Euro crowns but their campaign has been marred by allegations that goalkeeper David de Gea was involved in a sex scandal.