Top seeds Agnieszka Radwanska and Australian Open champion Angelique Kerber crashed out of the Madrid Open in the first round on Sunday in major setbacks ahead of Roland Garros.
Poland's Radwanska, the number one seed in the absence of Serena Williams, lost 6-4, 6-7 (3/7), 6-3 to the rejuvenated Dominika Cibulkova of Slovakia.
Radwanska, a semi-finalist in 2012 and 2014 in the Spanish capital, already knew the dangers posed by Cibulkova after her opponent came within a point of victory in their last meeting at Indian Wells in March.
Cibulkova, a former world number 10 and Australian Open runner-up, was always going to be a dangerous floater having won her fifth career title last time out in Katowice three weeks ago.
Second-seeded German Kerber was dumped out by the Czech Republic's Barbora Strycova 6-4, 6-2 with her defeat coming just a week after she had retained her title in Stuttgart.
However, Kerber was outplayed by world number 33 Strycova, who she had beaten 6-1, 6-1 in Miami back in March.
"I'd win four games in the matches we played before," said Strycova.
"I went into this match thinking I had nothing to lose. I mean, it can't be worse than Miami."
Losses for Radwanska and Kerber mean Garbine Muguruza is the highest seed left at the key clay-court event and the fourth-ranked Spaniard sealed her place in round two with a 6-2, 7-5 win over Anna Schmiedlova.
"Of course I was a little bit nervous and tense because the first matches are always complicated," said Muguruza, who is aiming to be the first Spanish woman to win the title in Madrid.
"In Madrid we have this extra pressure because I'm here at home. Perhaps you're a little bit more nervous because of that, but my first feeling is that I'm satisfied for winning the match."
Azarenka extends run
Former world number one Victoria Azarenka stretched her winning streak to 15 matches after winning in Indian Wells and Miami as she overcame some stiff first set resistence from Britain's Laura Robson to win 6-4, 6-2.
Azarenka could meet defending champion and fifth seed Petra Kvitova in the quarter-finals as the Czech had no trouble in disposing of Lara Arrubarrena 6-3, 6-2.
US Open finalist Roberta Vinci, the seventh seed, is out after losing in straight sets to Montenegrin Danka Kovinic 6-4, 6-2.
German Laura Siegemund continued her great start to the clay season after reaching the Stuttgart final as a qualifier as she dumped out ninth seed Svetlana Kuznetsova 6-0, 3-6, 6-3.
Eighth seed Carla Suarez Navarro came from a set down to beat Hungary's Timea Babos 3-6, 6-2, 6-3.
Ana Ivanovic also had to go the distance to see off Katerina Siniakova 6-3, 3-6, 6-4 while 2014 French Open runner-up Simona Halep, the sixth-seeded Romanian, swept past Japan's Misaki Doi 6-0, 6-3.
There was also a notable win for Swiss 10th seed Timea Bacsinszky who saw off Germany's Andrea Petkovic 6-4, 2-6, 6-3 despite only arriving in Spain at midnight having won the Rabat title on Saturday.
Also shrugging off jet-lag was Czech 11th seed Lucie Safarova, the champion in Prague on Saturday, who beat Coco Vandeweghe 7-6 (7/4), 6-1.
Australia's Samantha Stosur, beaten by Safarova in the Czech final 24 hours earlier, was also a winner, seeing off home player Sara Sorribes 6-1, 6-3.
Top Seeds Agnieszka Radwanska, Angelique Kerber Crash Out of Madrid Masters
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Top seeds Agnieszka Radwanska and Australian Open champion Angelique Kerber crashed out of the Madrid Open in the first round
Agnieszka Radwanska was seeded No. 1 at Madrid Masters after Serena Wiliams' exit due to flu.© AFP
Highlights
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Top seed Radwanska lost to Dominika Cibulkova in Rd 1
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Second-seeded German Kerber lost to Barbora Strycova
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Serena Williams has pulled out of the tournament due to flu