The Court of Arbitration for Sport (CAS) on Monday rejected India's appeal, filed after boxer Vikas Krishan was controversially eliminated from the London Olympics.
Vikas was declared the winner of his 69 kg bout against Errol Spence before International Amateur Boxing Federation (AIBA) overturned the decision in American's boxer.
A CAS statement said there was no provision for an appeal against AIBA's decision.
"There is no provision in the AIBA Technical and Competition Rules allowing for an appeal against the decision of the competition jury in relation to a protest. The decision of the competition jury is final and cannot be appealed," the CAS said.
"For this reason, your appeal letter cannot be considered and the appeal letter is hereby returned to you," the statement added.
P.K. Muralidharan Raja, India's acting chef de mission, said he was hurt with AIBA's decision.
"Although Team India feels very hurt at the reversal of decision of bout no.142 and declaring the opponent of Vikas Krishan to be the winner, with the approval and concurrence of acting President IOA Prof Vijay Kumar Malhotra and Secretary General IOA, the subject matter is being rested and not pursued any further at this competition as the protest is being considered non-maintainable," said Raja in a statement.