PV Sindhu knocked out after losing 19-21, 18-21 to Thailand's Nitchaon Jindapol in second round at Australian Open.
PV Sindhu beat Indonesia's Choirunnisa 21-14, 21-9 in a one-sided affair.
This is India's second consecutive defeat in the Sudirman Cup 2019 as they lost 2-3 to Malaysia on Tuesday.
India's hopes of reaching the knockout stage will now rest on their next match against home favourites China on Wednesday.
In the last edition at Gold Coast, Australia, India had reached the quarterfinals and have been seeded eighth in this edition.
Saina Nehwal was knocked out after losing 13-21, 23-21, 16-21 against third seed Akane Yamaguchi of Japan in her women's singles quarterfinal.
PV Sindhu, Saina Nehwal and Sameer Verma won their Round of 16 matches in straight sets.
PV Sindhu cruised through her opponent while Saina Nehwal and Sameer Verma had to work extra hard for their wins.
PV Sindhu lost in straight games to Nozomi Okuhara of Japan in the Singapore Open semifinals.
PV Sindhu took just 39 minutes to record a 21-13, 21-19 win over the world number 22.
PV Sindhu defeated Indonesia's Lyanny Alessandra Mainaky 21-9, 21-7 in 27 minutes to sail into the second round.
PV Sindhu lost the Malaysia Open second round match to Sung Ji Hyun, who had also knocked out Sindhu out from the All England Championships.
Kidambi Srikanth defeated China's Huang Yuxiang 16-21, 21-14, 21-19 to enter men's singles final in the India Open badminton tournament.
Kashyap will be up against Viktor Axelsen in Saturday's semi final, while Srikanth will face China's Huang Yuxiang.
Five singles players and four doubles pairs from India have entered the quarter-finals of the India Open, a BWF World Tour Super 500 tournament.
Subhankar Dey produced the biggest upset of the first day of the India Open, beating World No.9 and fourth seed Tommy Sugiarto of Indonesia.
PV Sindhu could have an advantage with the Japanese players not in action.