Fourth seeds Bhupathi and Mirnyi were scheduled to play against Americans John Isner and Sam Querry, who withdrew from the contest due an ankle injury to Isner.
Bhupathi and Mirnyi are yet to face a ball in the USD 24,30,000 hard court event as they had got a bye in the first round.
The Indo-Belarusian pair will next take on Polish pair of Mariusz Fyrstenberg and Marcin Matkowski, who edged out Bopanna and Qureshi in their second round match.
The Indo-Pak pair fought hard before losing 5-5 6-7(3) to the eighth seeds.
Third seeds Leander Paes and Czeck Lukas Dlouhy will play their second round match against America's Mardy Fish and Bahamas' Mark Knowles.
Somdev Devvarman had lost in the first round of the singles event.