The 30-year-old Frenchwoman, a former top-ranked player who finished the season No. 21, said she doesn't have the will to keep playing at the highest level.
"I don't want to train anymore," Mauresmo said after shedding tears about the decision. "I had to make a decision, which became evident in the last few months and weeks. When you grew older, it's more difficult to stay at the top."
Mauresmo, who won both of her Grand Slam titles in 2006, at the Australian Open and Wimbledon, said she was happy to leave on a good note after winning her 25th singles title _ her first in almost two years _ in Paris this season.
"It's a bit sad, but this is the right decision," Mauresmo said. "I was lucky enough to have an exceptional career and to experience very strong feelings on the court."
Mauresmo, who was the No. 1-ranked player in 2004, played her last match in the second round of this year's U.S. Open, losing to Aleksandra Wozniak of Canada 6-4, 6-0. She then pulled out of her last two tournaments of the year.
"It became very hard in build-up to the U.S. Open," Mauresmo said. "If I were able to enter the court, play and shine, of course I could continue, but to achieve this you need to put in such hard work. And I'm not capable of that."
Mauresmo said she has no regrets and feels very proud when she looks back at a career that started in 1993.
"I dreamt of this career, I dreamt of winning a Grand Slam title," she said. "I lifted trophies in every city in the world and I lived 10 magical and unbelievable years."
Mauresmo, who says she decided to play tennis after watching Yannick Noah win the 1983 French Open, became the first player from France _ male or female _ to reach the No. 1 spot on Sept. 13, 2004.
But she was never able to emulate Noah's feat of winning on the clay at Roland Garros, failing to go beyond the quarterfinals at the Grand Slam tournament in Paris, where she struggled to withstand the pressure in front of her home crowd.
Mauresmo, whose backhand delighted tennis pundits around the world, also won the Fed Cup with France in 2003 and the WTA Tour championship in 2005. She won the Olympic silver medal in Athens in 2004.
Asked about a possible comeback, the Frenchwoman said her decision was definitive.
"Even if I've learned to never say never," Mauresmo said. "The players you are thinking about stopped earlier than me before coming back."
Former No. 1-ranked player Justine Henin confirmed in October she'll make her return to the WTA Tour at the Brisbane International _ two weeks before the Australian Open. Kim Clijsters won the U.S. Open in September after coming back from two years in retirement.